ABSTRACT
In this study, Escherichia coli BL21 (DE3) was used as the host to construct 2 recombinant E. coli strains that co-expressed leucine dehydrogenase (LDH, Bacillus cereus)/formate dehydrogenase (FDH, Ancylobacter aquaticus), or leucine dehydrogenase (LDH, Bacillus cereus)/alcohol dehydrogenase (ADH, Rhodococcus), respectively. L-2-aminobutyric acid was then synthesized by L-threonine deaminase (L-TD) with LDH-FDH or LDH-ADH by coupling with two different NADH regeneration systems. LDH-FDH process and LDH-ADH process were optimized and compared with each other. The optimum reaction pH of LDH-FDH process was 7.5, and the optimum reaction temperature was 35 °C. After 28 h, the concentration of L-2-aminobutyric acid was 161.8 g/L with a yield of 97%, when adding L-threonine in batches for controlling 2-ketobutyric acid concentration less than 15 g/L and using 50 g/L ammonium formate, 0.3 g/L NAD+, 10% LDH-FDH crude enzyme solution (V/V) and 7 500 U/L L-TD. The optimum reaction pH of LDH-ADH process was 8.0, and the optimum reaction temperature was 35 °C. After 24 h, the concentration of L-2-aminobutyric acid was 119.6 g/L with a yield of 98%, when adding L-threonine and isopropanol (1.2 times of L-threonine) in batches for controlling 2-ketobutyric acid concentration less than 15 g/L, removing acetone in time and using 0.3 g/L NAD⁺, 10% LDH-ADH crude enzyme solution (V/V) and 7 500 U/L L-TD. The process and results used in this paper provide a reference for the industrialization of L-2-aminobutyric acid.
Subject(s)
Aminobutyrates , Metabolism , Escherichia coli , Genetics , Formate Dehydrogenases , Metabolism , Leucine Dehydrogenase , Metabolism , NAD , MetabolismABSTRACT
OBJECTIVE@#To explore the effects of different analgesia methods after UPPP.@*METHOD@#Ninety cases of patients uvulopalatopharyngoplasty were divided into 3 groups randomly, and 30 cases in each group. The group A was the blank control group without any analgesia measures. The cases in group B were treated with intramuscular injection of parecoxib sodium 40 mg after surgery immediately, and continued injecting 40 mg after 12 hours, 24 hours and 36 hours respectively. 100 mg tramadol replaced 40 mg parecoxib sodium in group C. The VAS scoring was performed after surgery 12, 24, 36, 48, 72, 96 hours in 3 groups, and we observed adverse reaction such as lethargy, nausea, vomiting, dizziness, skin rash and so on.@*RESULT@#The group B and C reduced the pain significantly compared with blank control group. The pain scores in group B were significantly decreased than that in group C (P<. 05).@*CONCLUSION@#The analgesic effect of parecoxib sodium after UPPP is significant and better than tramadol. It is worthy to use widely in clinical due to its better effect and less side effect.
Subject(s)
Humans , Analgesia , Methods , Analgesics , Therapeutic Uses , Injections, Intramuscular , Isoxazoles , Therapeutic Uses , Pain Measurement , Pain, Postoperative , Palate , General Surgery , Pharynx , General Surgery , Tramadol , Therapeutic Uses , Uvula , General SurgeryABSTRACT
ObjectiveTo observethe clinical efficacy of surround needling plus high-energy red light in treating herpes zoster. MethodSixty patients with herpes zoster were randomized into a medication group, a control group and a united group. The medication group was intervened by oral administration of medication, the control group was by surround needling plus electroacupuncture in addition to the medication, and the united group was by the medication, surround needling plus electroacupuncture, and the high-energy red light radiation. The effect on pain and the comprehensive therapeutic efficacy were evaluated on treatment day 5 and day 10.ResultThe efficacies in releasing pain in the control group and united group were significantly superior to that in the medication group on treatment day 5 (P0.05); on treatment day 10, the effects on releasing pain in the control group and united group were more significant than that in the medication group (P0.05); on treatment day 10, the efficacies in the control group and united group were significantly better than that in the medication group (P<0.01), and the efficacy in the united group was superior to that in the control group (P<0.05). ConclusionSurround needling plus high-energy red light can effectively reduce the neuralgia caused by herpes zoster, shorten the pain duration, improve the symptoms, andthus this combination is an effective and safe method.
ABSTRACT
Objective To evaluate the clinical effect of cytokine induced killer cellular immunotherapies ( CIK) on the patients with advanced lung cancer.Methods Peripheral blood mononuclear cell from 32 cases of patients with advanced lung cancer were isolated.Cells were cultured in DMEM supplemented with IFN-γ,IL-2,anti-CD3 monoclonal antibody and other cytokines,and transfused back into the patients in several times after 15 days of culture.Subsequently,we observed the size of tumor,the change of immunologic function by CIK treat-ment,the status of clinical symptoms,and the toxic and side effect.The clinical curative effect was also evaluated. Results The symptoms in 32 patients who received CIK therapy were reduced,and the side effects were light. Immunophenotype analysis showed that CD3 /CD4 coexpression were found in 34.4%patients,CD3 /CD8 co-expression in 68.2%,and CD3 /CD56 co-expression in 13.7% patients.CT images about 15 days after CIK cell transfusion showed that pleural effusion were decreased.Clinical evaluation effective rate was 56.0%,and disease control rate( CR +PR +SD) was 84.3%.Conclusion CIK cell immunotherapies of cancer patients can improve immunologic function of cancer patients and inhibit tumor growth and relieve the clinical symptom of patients,and improve the quality of life.